Investigating Low Temperature Acclimation and Cold Tolerance in Faba bean

Faba bean (Vicia faba L.) is an important leguminous crop. Winter sown faba bean has 10-30% higher yield potential compared to spring types, yet non-availability of highly tolerant winter varieties making its cultivation difficult in temperate climate. The aim of my project is to analyze the genetic mechanisms underlying cold tolerance. It comprise screening of inbreds at low temperatures to evaluate their survival and regrowth after frost incidents as well as investigate the effect of different acclimation conditions. I will establish a standard protocol for cold tolerance screening of faba bean for identifying suitable genetic resources for breeding programs. Subsequently, the generation of F1 test hybrids based on inbred lines with different trait components should enable to investigate the effect of heterosis for cold tolerance in faba bean. Furthermore, the genetic analyses will be complemented by analyses of gene expression using RNAseq and RT-qPCR and by metabolite studies that will confirm and the functional dynamics of cold tolerance in faba bean. This knowledge should enable breeders to breed winter faba beans in a more targeted way, which have a broad cultivation potential and thus contribute to the local production of plant-based protein.